Fig. 2: Nucleosome loss/retention associated with spermiogenesis occurs non-randomly in a large fraction of the genome. | Nature Communications

Fig. 2: Nucleosome loss/retention associated with spermiogenesis occurs non-randomly in a large fraction of the genome.

From: Epigenetic homogeneity in histone methylation underlies sperm programming for embryonic transcription

Fig. 2

a Relative abundance (left) and genome coverage (right) of nucleosomes and subnucleosomes in the sperm chromatin. The left pie chart reports the fraction of DNA fragments from Xenopus Laevis sperm corresponding to each type of particle; the right pie chart reports the fraction of genome covered by each type of particle. b Fraction of the genome with homogeneous particles composition. The bar graph indicates the percentage of the genome that possess nucleosomal or subnucleosomal structure across most sperm of the population sequenced (genome binned in 50 bp windows). c Fold enrichment (observed/random) over 1000 randomisations for homogeneous nucleosomes (left) or subnucleosomes (right) composition at the indicated genomic features; ***: empirical p value < 1e−3. Input data from two independent replicates were pooled. d PAM (partitioning around medoids) clustering of promoter (TSS +/−2 kb) according to enrichment for nucleosomes or subnucleosomes. e Model of nucleosomes and subnucleosomes distribution in sperm and spermatid. Source data related to a, b and d are provided as Source Data files.

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