Table 1 Cryo-EM and refinement statistics of the P. arsenaticum and the S. solfataricus pilus.
From: The structures of two archaeal type IV pili illuminate evolutionary relationships
Parameter | P. arsenaticum pilus | S. solfataricus pilus |
|---|---|---|
Data collection and processing | ||
Voltage (kV) | 300 | 300 |
Electron exposure (e− Å−2) | 50 | 58 |
Pixel size (Å) | 1.08 | 1.08 |
Final particle images (n) | 210,331 | 579,713 |
Helical symmetry | ||
Point group | C1 | C1 |
Helical rise (Å) | 5.26 | 4.97 |
Helical twist (°) | 101.69 | 104.57 |
Map resolution (Å) | ||
Map:map FSC (0.143) | 3.8 | 3.4 |
Model:map FSC (0.38) | 3.8 | 3.4 |
d99 | 3.9 | 3.6 |
Refinement and model validation | ||
Map-sharpening B-factor (Å2) | −140 | −104 |
Bond lengths RMSD (Å) | 0.005 | 0.006 |
Bond angles RMSD (°) | 1.004 | 0.986 |
Model:map RSCC | 0.87 | 0.85 |
Clashscore | 8.82 | 7.28 |
Poor rotamers (%) | 0.5 | 0 |
Ramachandran favored (%) | 95.63 | 95.73 |
Ramachandran outlier (%) | 0 | 0 |
MolProbity score | 1.78 | 1.70 |
Deposition ID | ||
PDB (model) | 6W8U | 6W8X |
EMDB (map) | EMD-21578 | EMD-21579 |
