Fig. 1: Schematic of the RIACS.

Suspended cells injected into the RIACS are focused by the hydrodynamic and acoustic focusers into a single stream, detected by the event detector, imaged by the ultrafast multicolor SRS microscope, analyzed by the real-time Raman image processor, and sorted by the dual-membrane push-pull cell sorter triggered by decisions made by the real-time Raman image processor composed of multiple FPGAs, CPUs, and a network switch, all on a 10-Gbps all-IP network for high-speed digital image processing and decision making. The entire process is operated in a fully automated and real-time manner. Supplementary Fig. 1 shows pictures of the major components of the RIACS.