Fig. 3: Th1-baised antigen recall response by SPy_2191-immunized mice splenocytes. | Nature Communications

Fig. 3: Th1-baised antigen recall response by SPy_2191-immunized mice splenocytes.

From: Cross-serotype protection against group A Streptococcal infections induced by immunization with SPy_2191

Fig. 3: Th1-baised antigen recall response by SPy_2191-immunized mice splenocytes.

Two weeks after the final immunization, splenocytes were extracted (n = 5), cultured under ex vivo conditions and stimulated with SPy_2191 antigen for 72 h. a–g Release of Th1-specific IFN-γ, TNF-α, and IL-2; Th2-specific IL-4, IL-6, and IL-10, and Th17-specific IL-17A cytokines, was measured in the extracellular culture supernatant. Two-tailed unpaired t-test was employed for calculating the significant difference in all the cases. Data are presented as mean values ± SEM. ****P < 0.0001.

Back to article page