Fig. 4: Impacts of Fer-1 and GPX4 expression in TMD-treated cells.
From: Vacancies on 2D transition metal dichalcogenides elicit ferroptotic cell death
a Effects of Fer-1 on MoS2 and WS2 induced cytotoxicity (n = 3 biologically independent cell samples). BEAS-2B and THP-1 cells were pretreated by 10 μM Fer-1 for 1 h, followed by 48 h incubation with 200 μg/mL WS2 and MoS2 (n = 3). MTS assay was used to assess the impacts of Fer-1 on WS2/MoS2-induced cell deaths. Data are presented as mean values ± SD. **p < 0.01 and ***p < 0.001 compared to cells without Fer-1 pretreatment by two-tailored Student t-test. b Assessment of GPX4 expression in BEAS-2B cells. After exposure to 200 μg/mL WS2 and MoS2 for 48 h, BEAS-2B cells were collected, washed and lyzed for Western blotting assay (left panel). The relative expression of GPX4 was assessed by the optical density of each band on representative Western blotting images (right panel, n = 3 independent experiments). Data are presented as mean values ± SD. *p < 0.05, compared to Ctrl cells by two tailored Student t-test.
