Fig. 6: Validation of ferroptosis in alveolar macrophages from lung tissue.
From: Vacancies on 2D transition metal dichalcogenides elicit ferroptotic cell death
a Representative Western blotting images of GPX4 from three independent experiments, b representative images of lysosomal release of Fe2+, and c lipid peroxidation in alveolar macrophages from four independent views. Animals pretreated with or without 50 μg/Kg Fer-1 for 2 h were exposed to 1 mg/Kg WS2 for 40 h (n = 6 mice). Then the BALF of animals were collected to extract alveolar macrophages. The cells were divided into three portions. One was lysed for Western blotting analysis, and the other two were stained by Image-iT lipid peroxidation staining kit (scale bar: 10 μm) to visualize nuclei (blue), reduced substrate (red) and oxidized substrate (green), or FeRhoNox-1 (scale bar: 10 μm) to observe lysosomal release of Fe2+.
