Fig. 1: Screening of Arabidopsis transcription factors to identify GAPC-binding proteins. | Nature Communications

Fig. 1: Screening of Arabidopsis transcription factors to identify GAPC-binding proteins.

From: Nuclear moonlighting of cytosolic glyceraldehyde-3-phosphate dehydrogenase regulates Arabidopsis response to heat stress

Fig. 1

a Mixture of the purified transcription factors. Proteins were purified by affinity chromatography and separated on a polyacrylamide gel. The gel was stained with Coomassie Brilliant Blue. Protein marker size is on the left. b Gel image of co-immunoprecipitation. Transcription factors co-immunoprecipitated with GAPC2-Flag or empty vector control (EV) were separated on a polyacrylamide gel and stained with Coomassie Brilliant Blue. Protein marker size is on the left. Positions of GAPC2, immunoglobulin G (IgG) heavy and light chains are on the right. c Mass spectrometry-based identification of NF-YC10. Trypsin-digested peptides from the immunoprecipitated samples were sequenced by LC-MS/MS. Shown here is NF-YC10 sequence with the unique peptides in red that were identified with probability >99%.

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