Fig. 4: Expression of heat-inducible genes in GAPC-altered Arabidopsis. | Nature Communications

Fig. 4: Expression of heat-inducible genes in GAPC-altered Arabidopsis.

From: Nuclear moonlighting of cytosolic glyceraldehyde-3-phosphate dehydrogenase regulates Arabidopsis response to heat stress

Fig. 4

Total RNA was extracted from 5-day-old seedlings of GAPC1-OE (a), GAPC2-OE (b), and gapc1gapc2 (c) treated at 37 °C for 5 h and quantitative RT-PCR was performed with gene-specific primers. Values are average ± S.D. and shown as fold change to WT (dashed line). P values indicate significant difference from WT determined by two-tailed student’s t test (n = 3 independent groups of >10 seedlings). Black dots represent individual data points. Hsf, heat shock transcription factor; Hsp, heat shock protein; At1g75860, DNA ligase; At4g36010, pathogenesis-related thaumatin superfamily protein; LFG4, LIFEGUARD4 (Bax inhibitor-1 family protein); EGY3, ETHYLENE-DEPENDENT GRAVITROPISM-DEFICIENT AND YELLOW-GREEN-LIKE3; CLPB3, CASEIN LYTIC PROTEINASE B3; FBS1, F-BOX STRESS INDUCED1; SAP10, STRESS-ASSOCIATED PROTEIN10; DREB2C, DEHYDRATION-RESPONSIVE ELEMENT BINDING PROTEIN 2C; At1g75960, AMP-dependent synthetase and ligase family protein; ACS7, 1-AMINO-CYCLOPROPANE-1-CARBOXYLATE SYNTHASE7.

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