Fig. 7: PP1 is a major regulator of 14-3-3 binding proteins. | Nature Communications

Fig. 7: PP1 is a major regulator of 14-3-3 binding proteins.

From: Dissecting the sequence determinants for dephosphorylation by the catalytic subunits of phosphatases PP1 and PP2A

Fig. 7

a Workflow of GFP-14-3-3β IP and subsequent MS analysis to identify 14-3-3-interacting proteins regulated by PP1. b Volcano plot of pairwise comparison between untreated/PP1c-treated samples (two-tailed Student’s t test, FDR 0.05). Proteins labeled in blue showed significant dissociation from 14-3-3 upon PP1c treatment and in orange represent SRSF proteins also identified in Table 1, 14–3–3 isoforms, and PP1 (added as recombinant protein). c Venn diagram showing overlap between the 56 proteins significantly dissociating from 14-3-3 upon PP1c treatment (but not binding GFP alone) compared to significantly dephosphorylated proteins (1967 p-sites on 1191 proteins) found in the PP1 phosphoproteomic experiments (t-test untreated vs. PP1 treated, see also Supplementary Fig. 4b and Supplementary Data 2, 3).

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