Fig. 7: TNC is correlated with LC3B and CD8+ T cells in clinical TNBC samples. | Nature Communications

Fig. 7: TNC is correlated with LC3B and CD8+ T cells in clinical TNBC samples.

From: Autophagy deficiency promotes triple-negative breast cancer resistance to T cell-mediated cytotoxicity by blocking tenascin-C degradation

Fig. 7

a, b Kaplan–Meier analysis of RFS based on TNC (201645_at) mRNA levels using the KM-plotter breast cancer database (http://kmplot.com/analysis). Auto select best cutoff was chosen in the analysis. All patients were stratified according intrinsic subtype as indicated (a). Basal patients were stratified according the known six Pietenpol subtypes (b). c The representative images of strong TNC staining in tumour cells (T) and weak staining in the matched adjacent normal cells (N) (Left). Quantitative IHC analysis of TNC staining of primary breast tumours and adjacent normal breast tissues was shown (n = 95). d The representative images for each IHC score of TNC staining in 160 primary TNBC tumours were shown. Score 1 represnted low TNC expression, Scores 2 and 3 represnted high TNC expression (left). Kaplan–Meier analysis of OS  for  patients.  All patients were stratified by expression of TNC (right). e The correlation of LC3B with TNC expression status in 158 primary TNBC tumours. Spearman's correlation coefficient r and P values were given at the bottom. f The correlation of CD8+ T-cell infiltration with TNC expression status in 150 primary TNBC tumours. Error bars represent mean ± SEM. The P value in c, f was determined by Wilcoxon matched-pairs signed rank test (two-sided). Survival curves in a, b, d were plotted by the Kaplan–Meier method and assessed using the log-rank test, and univariate Cox proportional hazards regression was carried out to identify HR and 95% CI.

Back to article page