Fig. 4: Orexin increases the GJ conductance in coupled SLD neurons.
a (Left) Double patch-clamp recordings in a pair of electrically connected SLD neurons in rat. (Right) Representative traces from this electrically connected pair of neurons showing that similar amplitude of voltage change in n1 (Vinjection) induced larger voltage response (Vresponse) in n2 in the presence of orexin-A (100 nM), and vice versa. b Group data showing that orexin-A bilaterally increased the coupling coefficient from cell 1 to cell 2 (left, two-sided Wilcoxon signed-rank test; z = 2.366, P = 0.0180) and cell 2 to cell 1 (right, two-sided Wilcoxon signed-rank test; z = 2.197, P = 0.0280) (n = 7 pairs of neurons). c Group data showing that the input resistances of cell 1 (left, two-sided Wilcoxon signed-rank test; z = 0.338, P = 0.735) and cell 2 (right, two-sided Wilcoxon signed-rank test; z = 1.690, P = 0.0910) were not affected by orexin-A (n = 7 pairs of neurons). d Group data showing that orexin-A bilaterally increased the GJ conductance from cell 1 to cell 2 (left, two-sided Wilcoxon signed-rank test; z = 2.366, P = 0.0180) and cell 2 to cell 1 (right, two-sided Wilcoxon signed-rank test; z = 2.366, P = 0.0180) (n = 7 pairs of neurons). Data represent mean ± SEM. *P < 0.05. Source data are provided as a Source Data file.
