Fig. 5: Adapted interaction modes are significantly weakened in the cross-interaction hANP32A:627-NLS(E). | Nature Communications

Fig. 5: Adapted interaction modes are significantly weakened in the cross-interaction hANP32A:627-NLS(E).

From: Molecular basis of host-adaptation interactions between influenza virus polymerase PB2 subunit and ANP32A

Fig. 5

Comparison of intermolecular contacts in hANP32A:627-NLS(K) (light orange), avANP32A:627-NLS(E) (dark orange) and hANP32A:627-NLS(E) (green). Three PREs are compared, a 631, b 587 and c 699. The comparison of PRE profiles due to the presence of a spin probe on residue 631 illustrates the reduced number and strength of contacts of the IDD, with the 627 domain in the hANP32A:627-NLS(E) complex compared to hANP32A:627-NLS(K). Residue 587 shows a similar effect. Comparison of PRE profiles due to the presence of a spin probe on residue 699 illustrates the lack of interaction with the linker-NLS region in the hANP32A:627-NLS(E) compared to avANP32A:627-NLS(E). The position of the hexapeptide region is identified with dotted vertical lines in c.

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