Fig. 6: YLL3 induced higher callus mineralization during fracture healing.
From: Identification of osteogenic progenitor cell-targeted peptides that augment bone formation
Female Prx1ERT-GFP mice were fractured at 2 months of age and received tamoxifen at 10 mg/kg for 3 days. YLL3 or YLL8 were given at 10 μg/kg, and hPTH (1–34) was given at 40 μg/kg, s.c., 5x/week for 10 or 21 days. a Representative callus images from the outer edge of the callus in indicated treatment groups at day 10 post fracture viewing under low power (4x) or high power (20x). Scale bar = 100 µm. b Representative microCT thickness mapping of the callus, callus bone volume, and bone mineral content from indicated treatment groups at day 10 post fracture. Columns represented individual values in each group and the error bars represented standard deviations. N = 5 animals group. *p < 0.05 between the treated groups versus PBS-treated group using one-way ANOVA followed by Dunnett’s multiple comparison post hoc test. c Representative callus images from the outer edge of the callus in indicated treatment groups at 21 days post fracture. Alizarin red was given at −1 day before euthanization. Scale bar = 100 µm. d Representative microCT thickness mapping of the callus, callus bone volume, and bone mineral content from indicated treatment groups at day 21 post fracture. Columns represented individual values in each group and the error bars represented standard deviations. N = 6 animals/group in PBS, YLL3, and YLL8-treated group and n = 5 in PTH-treated group. *p < 0.05 between the treated groups versus PBS-treated group using one-way ANOVA followed by Dunnett’s multiple comparison post hoc test.
