Fig. 7: Ceramide redistributes to ZIKV replication sites.

Huh7 cells were infected with ZIKV (MOI = 10). At 24 hpi, mock and infected cells were fixed, co-stained with the indicated antibodies, and visualized with Airyscan superresolution light microscopy. All images are representative of four independent experiments. a, b Cells were co-stained with antibodies against ceramide and ZIKV replication marker NS4B (a) or E protein (b). c, d Huh7 cells were transfected with SM-Eqt-GFP immediately following infection, then fixed, stained with antibodies against ceramide and NS4B (c) or E protein (d), and visualized as before. e, f Mock (e) and infected (f) cells were stained with antibodies against ceramide, NS4B, and the ER marker calnexin. g, h Pearson’s correlation coefficient was calculated for the indicated pairs of signals (n = 10 cells per condition pooled from four independent experiments; each dot represents r from a single field of view containing one or more cells). Thick central bar, mean; upper and lower bars, SD. Unpaired two-tailed Student’s t test. a–f Scale bar, 10 μm.