Fig. 2: Negative correlation between CircGRIA1 and Gria1 mRNA expression. | Nature Communications

Fig. 2: Negative correlation between CircGRIA1 and Gria1 mRNA expression.

From: CircGRIA1 shows an age-related increase in male macaque brain and regulates synaptic plasticity and synaptogenesis

Fig. 2: Negative correlation between CircGRIA1 and Gria1 mRNA expression.The alternative text for this image may have been generated using AI.

a Representative images of BASEscope in situ hybridization (ISH) showing circGRIA1 expression in PFC and hippocampus (CA1 and DG) of 10-Y and 20-Y male macaques. The images are representative of three independent experiments of each sample from 2 to 3 animals per age group. Red dots indicate circGRIA1 with nuclei counterstaining by hematoxylin. Scale bar, 50 μm. b Relative intensities of BASEscope ISH signals of circGRIA1 illustrated in (a) were quantified by use of Image J. Data are present as mean ± S.D. (n = 45–60 cells per age group; *p < 0.05, unpaired t-test). c Representative images of RNAscope ISH showing Gria1 expression in PFC and hippocampus of 10-Y and 20-Y male macaques. The images are representative of three independent experiment of each sample from 2 to 3 animals per age group. Green dots indicate Gria1 mRNA with nuclei counterstaining by DAPI. Scale bar, 100 μm. d Relative intensities of RNAscope ISH signals of Gria1 illustrated in (c) were quantified by use of Image J. Data are present as mean ± S.D. (n = 50–65 cells per age group; *p < 0.05, unpaired t-test). Source data are provided as a Source Data file.

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