Fig. 4: CircGRIA1 negatively regulates Gria1 mRNA expression.

a Representative images of BASEscope and RNAscope ISH showing circGRIA1 and Gria1 expression in hippocampal cultures of male fetal macaque at DIV14 and DIV28. The images are representative of three independent experiment of each sample from 2 to 3 fetal cultures per group. Red boxes indicate areas magnified. Scale bar, 100 μm. b Average intensities of circGRIA1 and Gria1 signals per neuron illustrated in (a) were quantified by use of Image J. Data are present as mean ± S.D. (n = 30–39 per group; *p < 0.01), p values were calculated using two-tailed Student’s t test or one-way ANOVA with Sidak’s correction for multiple comparisons. c Negative correlation between nascent circGRIA1 and Gria1 expression. Data are present as mean ± S.D. Each bar represents the average of three independent experiment; error bars denote S.D.; *p < 0.05, p values were calculated using two-tailed Student’s t test or one-way ANOVA with Sidak’s correction for multiple comparisons. d Protein extracts from DIV14, DIV28 male fetal macaque hippocampal neurons infected at DIV5 with viral circGRIA1, or siRNAs against circGRIA1 or control, were immunoblotted with GluR1 antibody. α-Tubulin was loading control. The blots represent three independent experiment of each hippocampal culture from 2 to 3 male fetal macaques. e Relative intensities of immunobloted signals of GluR1 illustrated in (d) were quantified by use of Image J. Data are present as mean ± S.D. Each bar represents the average of three independent experiment; error bars denote S.D.; n.s. no significance; *p < 0.05, p values were calculated using two-tailed Student’s t test or one-way ANOVA with Sidak’s correction or two-way ANOVA with Turkey’s correction for multiple comparisons. Source data are provided as a Source Data file.