Fig. 6: Knockdown of circGRIA1 increases synaptogenesis.

a Representative images of immunofluorescence (IF) showing levels of synapsin-I and PSD95 in hippocampal (CA1 and DG) of 10-Y and 20-Y male macaques. Ten-micron cryostat sections were immunostained with synapsin-I (green) and PSD95 (red) antibodies. Scale bar, 100 μm. b Representative images of IF showing levels of synapsin-I and PSD95 in hippocampus of 10-Y and 20-Y male macaques after in vivo knockdown of circGRIA1. Hippocampal ten-micron cryostat sections were immunostained with synapsin-I (green) and PSD95 (red) antibodies. Scale bar, 100 μm. c Relative intensities of synapsin-I and PSD95 immunostaining illustrated in (a) were quantified by use of Image J. Data are present as mean ± S.D. (n = 2–3 animals per age group). Each bar represents the average of three independent experiment; error bars denote S.D. (*p < 0.05, unpaired t-test). d Protein extracts from postmortem frozen PFC and hippocampal tissues of 10-Y and 20-Y male macaques were immunoblotted with synapsin-I and PSD95 antibodies. α-Tubulin was loading control. The blots represent three independent experiments of each sample from 2 to 3 animals per age group. e Relative intensities of immunobloted signals of synapsin-I and PSD95 illustrated in (d) were quantified by use of Image J. Data are present as mean ± S.D.; *p < 0.05, unpaired t-test. f Relative intensities of synapsin-I and PSD95 immunostaining illustrated in (b) were quantified by use of Image J. Data are present as mean ± S.D. (n = 2–3 animals per age group) (*p < 0.05, unpaired t-test). g Protein extracts from frozen hippocampal tissues of 10-Y and 20-Y male macaque were immunoblotted with synapsin-I and PSD95 antibodies. α-Tubulin was loading control. The blots represent three independent experiment of each sample from 2 to 3 animals per age group. h Relative intensities of immunobloted signals of synapsin-I and PSD95 illustrated in (g) were quantified by use of Image J. Data are present as mean ± S.D.; *p < 0.05, unpaired t-test, error bars denote S.D. Source data are provided as a Source Data file.