Fig. 2: Hematoxylin/eosin and immunohistochemical staining of tissues from EBOV-challenged rhesus macaques. | Nature Communications

Fig. 2: Hematoxylin/eosin and immunohistochemical staining of tissues from EBOV-challenged rhesus macaques.

From: Prior vaccination with rVSV-ZEBOV does not interfere with but improves efficacy of postexposure antibody treatment

Fig. 2

Histological analysis was performed on tissues collected during necropsy from all animals in the present study as well as historical controls. Representative H&E-stained tissue specimens (a–d, i–l, q–t) and IHC antibody labeled tissue specimens (e–h, m–p, u–x) are depicted ING. LN = inguinal lymph node. For IHC images, EBOV antigen staining (VP40 protein), if present, is shown in brown. For the rVSV-EBOV + MIL77 cohort (n = 5), images were collected from subject #VSV/mAb-2, and are representative of all animals in the cohort. For the rVSV-EBOV cohort (n = 5), images were collected from subject #VSV-1, and are representative of all animals in the cohort surviving challenge. For the MIL77 cohort (n = 5), images were collected from subject #mAb-4, and are representative of all animals in the cohort surviving challenge. Images from a historical control animal, subject #H-7, are representative of what was observed in the other historical controls (n = 12) and the control animal (n = 1) for this study. Animals from the rVSV-EBOV and MIL77 only cohorts which succumbed to disease exhibited histological lesions and antigen labeling comparable to control animals. All images were captured at ×20 magnification.

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