Fig. 2: SARS-CoV-2 proteome microarray layout and quality control.
From: SARS-CoV-2 proteome microarray for global profiling of COVID-19 specific IgG and IgM responses
a There are 14 identical subarrays on a single microarray. A microarray was incubated with an anti-6xHis antibody to demonstrate the overall microarray quality (green). One subarray was shown. The proteins were printed in quadruplicate. The triangles indicate dilution titers of the same proteins. b Representative subarrays probed with sera of a COVID-19 convalescent and healthy control. The IgG and IgM responses were shown in green and red, respectively. c, d The correlations of the overall IgG and IgM signal intensities between two repeats probed with the same serum. Proteins (n = 93) on the microarray were examined. e Statistics of the Pearson correlation confidence among repeats probed with the same serum. Two serum samples from the convalescent group was examined in three independent experiments. NC negative control, PC positive control; 0.1, 0.2, 0.25, and 0.5 indicate the concentration of these proteins for microarray printing. T Tao Lab, B Hangzhou Bioeast biotech. Co.,Ltd., K Healthcode Co., Ltd., S Sanyou biopharmaceuticals Co.,Ltd., W VACURE l Biotechnology Co.,Ltd., Y Sino biological Co.,Ltd. Expression system: (1) E. coli: All proteins from Tao Lab (T), N Protein _S, N Protein_W; (2) Cell-free: All proteins from Healthcode Co., Ltd. (K), (3) Mammalian: S1_B, S1_S, S-RBD_S, S-RBD_Y.
