Fig. 9: BRD4 inhibition leads to changes in the percentages of S and G2/M phase cells. | Nature Communications

Fig. 9: BRD4 inhibition leads to changes in the percentages of S and G2/M phase cells.

From: BRD4 prevents the accumulation of R-loops and protects against transcription–replication collision events and DNA damage

Fig. 9

Representative images and quantification of flow cytometry cell cycle plots in a HeLa, b HCT116, and c RNase H-inducible HeLa cells following 48 h of JQ1 treatment. Data presented as mean ± SEM (n = 3 separate experiments). Significance assessed using ANOVA followed by Sidak’s test in a (%SDMSO vs JQ1 ***Adjusted P = 0.0002; % G2/MDMSO vs JQ1 ***Adjusted P = 0.0004); b (%SDMSO vs JQ1 ***Adjusted P = 0.0003; % G2/MDMSO vs JQ1 *Adjusted P = 0.0188), and c (%SDMSO RNase H1 negative vs JQ1 RNase H1 negative ***Adjusted P = 0.0008; %G2/MDMSO RNase H1 negative vs JQ1 RNase H1 negative *Adjusted P = 0.0155). d Growth curves of RNase H-inducible HeLa cells in the absence (−RNase H) or presence (+RNase H) of DMSO or JQ1. Data presented as mean (n = 2 independent experiments). Please refer to Supplementary Fig. 12 for flow cytometry gating strategy. Source data are provided as a Source data file.

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