Table 1 FasR association to DNA, and dose-dependent inhibition by effector ligands.

From: Mycobacterium tuberculosis FasR senses long fatty acyl-CoA through a tunnel and a hydrophobic transmission spine

 

FasRwt

FasRL98A

FasRF123A

Binding to DNA (PfasMT)

 appKD (nM)

83 ± 11

155 ± 18

39 ± 6

C20-CoA dose response

 IC50 (nM)

368 ± 105

634 ± 100

5269 ± 1067

 appKi (nM)

42

152

571

C16-CoA dose response

 IC50 (nM)

5415 ± 1047

ND

ND

 appKi (nM)

589

ND

ND

  1. Comparison of FasRwt vs mutants FasRL98A and FasRF123A that uncouple the allosteric effect.
  2. appKD and IC50 values are reported as the average of at least three independent experiments ± one standard error of the mean.
  3. ND: not detectable.
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