Fig. 1: Phage DNAs organize developmental processes into subcellular locations during infection.
From: Emerging heterogeneous compartments by viruses in single bacterial cells
a Combination of phage processes results in decision-making. Lytic decisions reported by a D-mNeongreen translational fusion and lysogenic decisions reported by a cI-mKO2 transcriptional fusion. b SeqA system detects single molecules of phage DNA. Methylated phages infect dam− cells. Phage DNA is bound by SeqA-mKO2 proteins. Only phage DNA retaining methylation is labeled. c DnaB is an essential DNA replication resource. DnaB-mTurquoise2 fusion protein reports localization of DnaB. d Tet system detects replicated phage DNAs. Phage DNA bearing tetO arrays is labeled by TetR-mCherry binding. e Representative infected cell with reporters described in a–d undergoes lytic development. Representative cells in e and g chosen from three independent infection experiments. * indicates contrast is adjusted for each time point for clarity. DnaB and replicated DNA fixed contrast images are shown in Supplementary Fig. 2b–e. All scale bars in this figure are 2 μm. f Kymograph of the cell in e. Explanations for data analysis in Supplementary Fig. 1 and Supplementary Discussion. Fluorescence is normalized to the population maximum. g Representative infected, lytic cell with two subcellular areas of development. h Kymograph of the cell in g. Fluorescence is normalized to the population maximum. i DnaB heat maps for lytic cells at their DnaB appearance time point are arranged by the position of DnaB. Cell to the left of i describes how location is represented for i–l. Fluorescence of each cell is normalized to its own peak brightness for i–l. n = 91 cells for i–l. j SeqA heat maps for lytic cells are arranged in the same order and time points as in i to compare SeqA and DnaB. k TetR heat maps for lytic cells at their TetR cluster appearance time point are arranged by the position of TetR. l DnaB heat maps for lytic cells are arranged in the same order and time points as in k to compare DnaB and TetR. Source data are provided as a Source Data file.
