Fig. 2: Organization of multiple heterogeneous subcellular areas during phage induction.
From: Emerging heterogeneous compartments by viruses in single bacterial cells
a Schematic for the spatial organization of phage development after induction. Lambda prophage is integrated into the bacterial chromosome, and different copies of the chromosome locate to different areas of the cell during growth. Induction of lysogens forces phages to develop in different areas of the cell. The prophage bears the gpD-mNeongreen lytic reporter and carries a tetO array. The cell harbors a TetR-mCherry plasmid and a DnaB-mTurquoise2 reporter. b Overlay images of lysogens after induction. At 0 min the cells were not yet induced (*indicates that the contrast is adjusted for each time point shown for clarity). All scale bars in this figure are 2 μm. c, d Intracellular areas of phage DNA and DnaB form. Histograms of the number of DnaB (c) and replicated DNA clusters (d) are shown for each time point. e Phage DNA replication varies intracellularly. For cells with more than one DNA cluster, the standard deviation of the size of the clusters is represented in boxplots for each time point, as a measure of intracellular phage DNA variability. The median is indicated by the dot at the center of the box, the box bounds the interquartile range of the data, the whiskers span the range of the data excluding the outliers, and the outliers are indicated as individual points. The approximate limit of our resolution is around 250 nm. Source data are provided as a Source Data file.
