Fig. 3: Bacterial DNA physically separates different intracellular phactories.
From: Emerging heterogeneous compartments by viruses in single bacterial cells
a Detection of phage and bacterial DNA locations. Phage carries the Tet reporter as in Fig. 1d and the D-mTurquoise2 lytic reporter. Cell carries a lacO array at its attB locus and bears a plasmid expressing both TetR-mCherry and LacI-EYFP to label phage and bacterial DNA. b, d, f Representative lytic cells with different bacterial DNA interactions. Phage DNA will push (b, 108/179 cells), spread (d, 56/179 cells), or squeeze (f, 15/179 cells) bacterial DNA as phage DNA expands. Representative cells chosen from four independent infection experiments. All scale bars in this figure are 2 μm. c, e, g Kymographs corresponding to the cells in b, d, f. Fluorescence is normalized to the population maximum. h Spatial distribution of bacterial DNA in lytic and non-lytic cells differs. In non-lytic cells, the locations of attB for three time points prior to cell division (orange), and in lytic cells, the location of the attB marker for three time points prior to cell lysis (blue) represent the location preference of bacterial loci in different developmental paths. The cell below shows how locations are represented. n = 653 data points for lytic and 382 data points for non-lytic categories. i, j Expanse of phage DNA pushes bacterial DNA. The sizes of phage DNA in each lytic cell that pushes bacterial DNA are shown as violin plots (i). The maximum (nearest to mid-cell) location of attB of the cells in i are shown as violin plots (j). Cell on the right shows how locations are represented for i, j. Cells were oriented such that the TetR clusters were all aligned toward one direction. In the violin plots, the solid line represents the median, and the dashed lines mark the bounds of the interquartile range of the data. Source data are provided as a Source Data file.
