Fig. 4: Structure of A320V and channel opening.
From: Structural mechanism for gating of a eukaryotic mechanosensitive channel of small conductance
a Cryo-EM density, contoured at 5.0 σ, for the A320V mutant. Each subunit is in a unique color. b Structure of A320V. The dimension of the channel is indicated. c The wide-open pore of A320V. d The pore profiles of the closed wild type (blue) and the open A320V mutant (red). e, f Structures of a single subunit for the wild type (e) and the A320V mutant channels (f). The C-terminal extramembrane domain in the mitochondrial matrix is colored in gray and the transmembrane region is colored in magenta. Cryo-EM densities, contoured at 7.0 σ, are shown for TM5. g, h Orthogonal views of the transmembrane domains of the wild-type MSL1 (g) and the A320V mutant (h).
