Table 1 Cryo-EM data collection, refinement and validation statistics.
From: Structural mechanism for gating of a eukaryotic mechanosensitive channel of small conductance
| Â | AtMSL1 in detergents (EMDB: 21444) (PDB: 6VXM) | AtMSL1 A320V in detergents (EMDB: 21445) (PDB: 6VXN) | AtMSL1 in nanodiscs (EMDB: 21447) (PDB: 6VXP) |
|---|---|---|---|
Data collection and processing | |||
Magnification | 105,000 | 105,000 | 105,000 |
Voltage (kV) | 300 | 300 | 300 |
Electron exposure (e–/Å2) | 62 | 62 | 62 |
Defocus range (μm) | −1.0 to −2.5 | −1.0 to −2.5 | −1.0 to −2.5 |
Pixel size (Ã…) | 1.1 | 1.1 | 1.1 |
Symmetry imposed | C7 | C7 | C7 |
Initial particle images (no.) | 936,130 | 428,889 | 457,683 |
Final particle images (no.) | 158,172 | 52,883 | 23,077 |
Map resolution (Ã…) | 3.06 | 2.96 | 3.39 |
    FSC threshold | 0.143 | 0.143 | 0.143 |
Map resolution range (Å) | 2.5–6.0 | 2.5–6.0 | 3.0–7.0 |
Refinement | |||
Initial model used (PDB code) | 5AJI | This study | This study |
Model resolution (Ã…) | 3.0 | 3.1 | 3.5 |
    FSC threshold | 0.5 | 0.5 | 0.5 |
Model resolution range (Ã…) | 2.7 | 2.7 | 3.1 |
Model composition | |||
    Non-hydrogen atoms | 15,064 | 14,385 | 14,819 |
    Protein residues | 1939 | 1862 | 1939 |
    Ligands | 21 | 7 | 0 |
B factors (Ã…2) | |||
    Protein | 36.2 | 93.1 | 73.6 |
    Ligand | 20 | 111 | N/A |
R.m.s. deviations | |||
    Bond lengths (Å) | 0.006 | 0.008 | 0.008 |
    Bond angles (°) | 0.763 | 1.283 | 1.126 |
Validation | |||
    MolProbity score | 1.57 | 1.28 | 1.64 |
    Clashscore | 5.58 | 5.31 | 6.59 |
    Poor rotamers (%) | 0 | 0 | 0 |
Ramachandran plot | |||
    Favored (%) | 96.1 | 98.45 | 95.94 |
    Allowed (%) | 3.9 | 1.55 | 4.06 |
    Disallowed (%) | 0 | 0 | 0 |
