Fig. 2: VEGFR2 signaling is critical for vascular regeneration in dura mater and brain after PTI.
From: VEGFR2 signaling drives meningeal vascular regeneration upon head injury
a Representative images of VEGFR2 upregulation in the surrounding BVs of putative injury areas (white dotted-lined circle or line) at D3 in adult mice. Similar findings were observed in four mice from two independent experiments. Scale bars, 500 µm. b Diagram depicting generation of VEGFR2iΔEC, Tie2iΔEC, or Dll4iΔEC mice and EC-specific deletion of VEGFR2, Tie2, or Dll4 in 8-week-old mice by i.p. injections of tamoxifen, PTI, and their analyses at 1 week after PTI. c–j Representative images and comparisons of CD31+BVs of putative injury areas (white dotted-lined circle or line) in the dura mater and brain at D7 in control and VEGFR2iΔEC, Tie2iΔEC, or Dll4iΔEC mice. Scale bars, 500 µm. Each dot indicates a value from one mouse and n = 7 (Dura mater Tie2iΔEC), n = 4 (Brain controls), n = 5 (all other groups) mice/group from two independent experiments. Vertical bars indicate mean ± SD. *P < 0.05, **P < 0.01 versus control by two-tailed Mann–Whitney U test.
