Fig. 6: VEGFR2, but not Tie2, signaling is required for maintaining dural vascular homeostasis.
From: VEGFR2 signaling drives meningeal vascular regeneration upon head injury
a Diagram depicting generation of VEGFR2iΔEC or Tie2iΔEC mice and EC-specific deletion of VEGFR2 or Tie2 at 8 weeks old of age and their analyses 4 weeks later. b Representative images of CD31+BVs (upper panels) and distributions of LYVE1+ (middle and lower panels) and F4/80+ macrophages (lower panels) in dura mater of control, VEGFR2iΔEC and Tie2iΔEC mice. Scale bars, 1 mm. Each white-lined box is magnified in middle and lower panels. Scale bars, 200 µm. c Comparisons of densities of CD31+BVs, LYVE1+macrophages and F4/80+ macrophages in control and VEGFR2iΔEC or Tie2iΔEC mice. Each dot indicates a value from one mouse and n = 5 (Tie2 control), n = 6 (Tie2iΔEC), n = 8 (all other groups) mice/group from three independent experiments. Vertical bars indicate mean ± SD. n.s., not significant or ***P < 0.001 versus control by two-tailed Mann–Whitney U test. MFI, mean fluorescence intensity. d, e Representative images of CD31+BVs in brain of control, VEGFR2iΔEC, and Tie2iΔEC mice. Scale bars, 200 µm. Comparisons of vascular density between control and VEGFR2iΔEC or Tie2iΔEC mice. Each dot indicates a value from one mouse and n = 5 (Tie2 control), n = 6 (Tie2iΔEC), n = 8 (all other groups) mice/group from three independent experiments. Vertical bars indicate mean ± SD. n.s., not significant versus control by two-tailed Mann–Whitney U test.
