Fig. 8: Rapid recovery of BV basement membrane in the injured dura mater but not in the injured brain after PTI.

a, b Representative images and comparisons of distributions of collagen IV along CD31+BVs in putative injury area (white dotted-lined circle) at indicated days in the dura mater of adult mice after PTI. Empty arrowheads indicate broken alignment of collagen IV. Scale bars, 200 µm. n = 4 mice/group from two independent experiments. Mean fluorescence intensity (MFI) of collagen IV per MFI of CD31 in the putative injury area at S14 is regarded as 100%. Dots and error bars indicate mean ± SD. **P < 0.01 versus S14 and ^##P < 0.01 versus D1 of collagen IV by Kruskal–Wallis test. c, d Representative images and comparisons of distributions of collagen IV and CD31+BVs in putative injury area (white dotted line) at indicated days in the brain of adult mice after PTI. Scale bars, 500 µm. Each indicated box area is magnified and arrayed in the right panels. Empty arrowheads indicate increased collagen IV in perivascular areas, whereas white arrowheads indicate collagen IV accumulation in avascular areas. n = 4 mice/group from two independent experiments. MFI of collagen IV or MFI of CD31 in the putative injury area at S14 is regarded as 100%. Dots and error bars indicate mean ± SD. **P < 0.01 versus S14 by Kruskal–Wallis test.