Fig. 4: Mutagenesis analyses.
From: Structure of an antagonist-bound ghrelin receptor reveals possible ghrelin recognition mode
Mutagenesis analyses of the polar amino acids of ligand-binding pocket (a–d) and the five phenylalanines forming the crevasse (e–i). Receptor activities were examined by intracellular Ca2+ concentration assay. EC50 values are means ± s.e.m. (n = 4).
