Fig. 4: Cav-1 promotes mitochondrial turnover. | Nature Communications

Fig. 4: Cav-1 promotes mitochondrial turnover.

From: Caveolin-1-mediated sphingolipid oncometabolism underlies a metabolic vulnerability of prostate cancer

Fig. 4: Cav-1 promotes mitochondrial turnover.The alternative text for this image may have been generated using AI.

a Representative images of mitochondria and lysosomes in PC-3M and LNCaP cells transfected with CellLight Lysosome-GFP (lysosomal associated membrane protein 1) and CellLight Mitochondria-RFP (leader sequence of E1 alpha pyruvate dehydrogenase). b Uptake of C11 TopFluor-SM in PC-3M cells following knockdown of CAV1. c Staining for mitochondria (CellLight Mitochondria-RFP (leader sequence of E1 alpha pyruvate dehydrogenase) and lysosomes (CellLight Lysosome-GFP (lysosomal associated membrane protein 1)) in PC-3M cells following knockdown of CAV1. d Co-staining for CAV1 (FITC) and mitochondrial potential/reactive oxygen species (MitoTracker Red CMXRos31) in PC-3M cells following knockdown of CAV1. e Intracellular levels of reactive oxygen species assessed via CellROX Deep red in PC-3M cells following knockdown of CAV1. All images were captured via confocal and widefield microscopy using live cell imaging techniques. Scale bars represent 50 µm; intensity scale bars are provided next to each figure. Digital image acquisition parameters and look up table mappings were uniformly set for all images within each respective group and are indicated to the right of each image group.

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