Fig. 2: The copy numbers of genes related to antioxidative functions and the transcriptional pattern of SOD genes.
a Copy numbers of SOD, CAT, LOX, TYR, and TRX genes in algal genomes. The dendrogram was generated based on the phylogenetic tree constructed based on the 424 single-copy orthologous gene sets identified from P. yezoensis and the other 17 published algal genomes. The expanded orthogroups related to antioxidative functions are shown in different colors. The size of the circle represents gene copy number. P values are listed at the bottom of each column. b Transcriptional level of SOD paralogs in the two life phases. Columns and vertical bars represent the average FPKM values of SOD genes in individual life phase and SD (blue: gametophyte; red: sporophyte; n = 4 biological independent samples), respectively. Red and blue stars indicate the gam-specifically transcribed and spo-specifically transcribed genes, respectively. c Heatmap showing the fold change of SOD paralog transcripts in response to osmotic stress. Fold change was calculated as log2(FPKM(x% of water loss or rehydration)/FPKM (ctrl)). Percentages above the heatmap denote the degree of water loss and 80%-re and 50%-re denote the rehydrated samples after 80 and 50% of water loss, respectively. WL water loss.
