Fig. 2: Zuo1 binds G4 motifs genome-wide and support G4 formation.
From: Zuo1 supports G4 structure formation and directs repair toward nucleotide excision repair
a IGV Genome Browser screenshot. Illustration of two 7.5 kb regions: top Chr XIII location 246,726 to 254,317 (G4_1) and bottom Chr X location 388,395 to 395,967 (G4_5). Zuo1-binding sites, location of G4 motifs and annotated genes are illustrated below. These regions were also among the regions tested in the qPCR, primers are indicated G4_1 and G4_5 (black track). b Genome-wide overlap of the Zuo1 peaks with G4 motifs as predicted by4. The black dotted line indicates a highly significant overlap p = 0.0001. c Southern–western combination to detect G4 structures in wildtype (wt), zuo1Δ and Zuo1-oe cells. Different amounts of genomic DNA were spotted on a membrane (2, 1, 0.5, and 0.25 µg), incubated with 2 µg/ml of BG4 antibody and detected by chemiluminescence. d BG4-ChIP analysis followed by qPCR of G4 levels in wt, zuo1Δ, and Zuo1-oe cells. Plotted are the means of n = 5 biologically independent experiments, which were normalized to wildtype. Error bars present ±SEM. Significance was calculated based on one-sided Student’s t-test. Asterisks indicate statistical significance in comparison with wildtype. *p < 0.05, ** p < 0.01, ***p < 0.001, ****p < 0.0001.
