Fig. 3: Zuo1 function at G4 has a positive effect on cellular fitness.
From: Zuo1 supports G4 structure formation and directs repair toward nucleotide excision repair
a–d Monitoring of growth changes in different yeast cells a, c Growth curves in liquid media were performed and doubling times (minutes) were calculated using the indicated yeast strains. Plotted are the means of n = 3 biologically independent experiments. Error bars present ±SEM. Significance was calculated based on one-sided Student’s t-test. Asterisks indicate statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001. b, d A serial dilution of yeast cells was spotted on rich media with and without 10 µM PhenDC3. Growth changes and sensitivity of n = 3 biologically independent experiments were monitored by colonies formation. e, f ChIP analysis followed by qPCR that monitored the binding of either Sgs1 (e) or Pif1 (f) to seven Zuo1 targets (G4_1–7) and two controls (NC_1,2) was monitored after ChIP and qPCR. ChIP was performed in wildtype and zuo1Δ cells. Presented data show fold decrease zuo1Δ/wt ± SEM. For all experiments, the means of three biological replicates were plotted. Significance was calculated based on one-sided Student’s t-test. Asterisks indicate statistical significance in comparison with wildtype cells. *p < 0.05, **p < 0.01, ***p < 0.001.
