Fig. 4: Zuo1 mediates NER pathway recognition at G4 sites.
From: Zuo1 supports G4 structure formation and directs repair toward nucleotide excision repair
ChIP and qPCR analysis of different repair proteins in wildtype and zuo1Δ cells. All qPCRs were performed at seven Zuo1 targets (G4_1–7) and two controls (NC_1, 2). Presented data show fold decrease zuo1Δ/wt ± SEM of n = 3 biologically independent experiments. a ChIP and qPCR of Rad50-Myc. b ChIP and qPCR of Ku70-Myc c ChIP and qPCR of Apn1-Myc d ChIP and qPCR of Rev1-Myc e ChIP and qPCR of Rad23-Myc f ChIP and qPCR of Rad23-Myc in the presence of 10 µM of PhenDC3. All plotted results were based on the average of at least three independent experiments ± SEM. Significance was calculated based on one-sided Student’s t-test. Asterisks indicate statistical significance in comparison with wildtype cells under the same experimental conditions. *p < 0.05, **p < 0.01. g Yeast cells were grown on liquid media in the presence or absence of PhenDC3, irradiated with 15 J m−2 UV light (254 nm) and spotted in different concentrations of rich media. Growth changes and sensitivity were monitored by colony formation.
