Fig. 5: Patient tumor profile closely matches 005 mouse tumor.

a Population analysis of cells isolated from tumor tissue excised during surgery from GBM patients and mouse tumor samples as previously described. Murine populations were defined as following: B cells (B220+ CD45+), Ly6G+ Macs/Neutrophil (Ly6C+ Ly6G+ CD11b+ CD45+), conventional CD4 (CD3+ CD4+ Tim3− Lag3− CD25− CD45+), regulatory T cells (CD4+ CD25+ KLRG1+ CD103+), CD103+ CD8 (CD3+ CD45+ CD8+ CD103+), conventional CD8+ (CD3+ CD8+ CD45+), eosinophils (SiglecF+ CD11b+ MHCII−), DCs (CD11c+ CD64+ CD86+ MHCII+ CD45+), resident macrophages (CD45+ CD11b+ MHCII+ Ly6C−), infiltrating macrophages (CD45+ CD11b+ MHCII+ Ly6C+), resting microglia (CD11b low CD45 low MHC-II low), activated microglia (CD11b low CD45 low MHC-II high), NK cells (NK1.1+ CD49b+). GBM patient populations were defined as B cells (CD19+ CD20+), Ly6G+ Macs/Neutrophil (CD15, CD66b), conventional CD4 (CD3+ CD4+ CD25− CTLA4−), regulatory T cells (CD4+ CD25+ CTLA4+) CD103+ CD8 (CD3+ CD45+ CD8+ CD103+), conventional CD8+ (CD3+ CD8+ CD45+), eosinophils (Siglec8+, CD68+), DCs (CD11c++ MHCII++ CD45+), resident macrophages (CD45+ CD11b+ CD14+ CD68+ CD172ab+ CCR2−), infiltrating macrophages (CD45+ CD11b+ CD14+ CD68+ CD172ab+ CCR2+), resting microglia (CD11b low CD45 low MHC-II low), activated microglia (CD11b low CD45 low MHC-II high), NK cells (NKp30+, CD16+). b Abundance analysis plotted as % of cells for populations defined in a. Data represented as average ± SE (n = 3 mice/group and five patient samples/group; two independent experiments). Two-sided Student’s t test with Holm–Sidak corrections for multiple comparisons was applied. *p ≤ 0.05, **p ≤ 0.005. c Spearman’s rank correlation coefficient (R) was calculated for GBM patients with each of the mouse tumor models for n = 3 mice/group and five patient samples. Data representative of two independent experiments.