Fig. 1: Cryo-EM structure of the human mitochondrial 55S·EF-G1mt·GMPPCP Class III complex.

a Segmented cryo-EM densities, showing 28S (semitransparent yellow), 39S (semitransparent blue), EF-G1mt (red), P-site tRNAmt (green), fragmented E-site tRNAmt (brown), and small segment of mRNA (purple), as viewed from the subunit-subunit interface side. b Atomic model of components within the complex, colored as in panel a. Landmarks on the 28S subunit: h head, b body. Landmarks on the 39S subunit: CP central protuberance, Sb uL11m stalk base. c Structure of EF-G1mt displaying its five domains and the bound GMPPCP molecule, with a color-coded bar diagram depicting domain organization of EF-G1mt. d Interaction between the C-terminal domain (CTD, blue) of uL12m and G′ subdomain (orange) of EF-G1mt.