Fig. 4: Interactions of tRNAsmt and EF-G1mt with mito-specific proteins and mito-specific segments in homologous proteins. | Nature Communications

Fig. 4: Interactions of tRNAsmt and EF-G1mt with mito-specific proteins and mito-specific segments in homologous proteins.

From: Structures of the human mitochondrial ribosome bound to EF-G1 reveal distinct features of mitochondrial translation elongation

Fig. 4: Interactions of tRNAsmt and EF-G1mt with mito-specific proteins and mito-specific segments in homologous proteins.The alternative text for this image may have been generated using AI.

a Interactions of the A (purple), P (green), and E (brown) tRNAsmt with mito-specific proteins, PSF (orange), mL48 (blue), mL64 (pink), and mito-specific insertion/extension to homologous proteins uL11m (dark cyan) and uL16m (yellow). The A-site tRNAmt position in its A/A state is derived from the 55S bovine (Bos taurus) map (see Supplementary Fig. 8). b Dynamics of PSF, which has moved by ~4 Å towards the P-site tRNA while interacting with the D and T loop regions P-site tRNA (P/P state) in the 55S·EF-Gmt·GMPPCP complex, from its position in the vacant human mitoribosome (gray)2. c Structure of the C-terminal region (A160-E192, darker pink) of mL64 derived from Class III map (blue). d Interaction between the mito-specific segment of uL11m (dark blue) with the domain IV of EF-G1mt (red), involving a hydrogen-bond between uL11m’s C-terminal residue K192 and E562 from domain IV. Thumbnails to the left of panels a, b, and d depict overall orientation of the 39S and 55S mitoribosomes. Landmarks on thumbnails: CP central protuberance, L1 uL1m stalk, Sb uL11m stalk base of the 39S LSU, h head, b body of the 28S SSU.

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