Fig. 4: Icv FGF1 induces a population of differentiating oligodendrocytes.
a UMAP clustering of cells in the oligodendrocyte lineage from the Day 5 scRNA-seq dataset. Cell type annotations were inferred from a published dataset35. b Dots represent mean number of cells per animal and lines represent mean ± SEM. Unpaired t test, two tailed, *p < 0.022, **p < 0.0074 (Bonferroni adjusted; n = 6 mice/group); ns not significant. Source data for each subcluster are provided as a Source data file. c UMAP of cells in the oligodendrocyte lineage labeled according to maturation in Monocle-derived pseudotime. d UMAP of cells expressing Gpr17, a maturing oligodendrocyte marker. e RNAScope detection of Gpr17 expression in the hypothalamus of Lepob/ob mice 5 days after icv FGF1 or vehicle. f Quantification of Gpr17+ cells from the ARC area (2 sections per animal, icv vehicle (n = 4) and icv FGF1 (n = 5)), mean ± SEM. Unpaired t test, two tailed, *p = 0.005 (not adjusted). Source data are provided as a Source data file. NFOL newly formed oligodendrocyte, MFOL myelin-forming oligodendrocyte, MOL1 myelinating oligodendrocyte.
