Fig. 7: FGF1-induced long-term changes in the hypothalamus.
a Number of DEGs (|log2 fold change| > 0.5; FDR < 0.05) from bulk RNA-sequencing (bRNA-seq) of hypothalami from Lepob/ob mice injected with icv FGF1 or vehicle and harvested Days 1 (FGF1 n = 10, vehicle n = 4), 5 (FGF1 n = 9, vehicle n = 7), and 42 (n = 5 mice/group) after injection. Control vehicle mice were pair-fed to FGF1-injected mice. b Rank–rank hypergeometric overlap maps of the degree of overlapping genes between Day 1 and Day 5 (top) and between Day 5 and Day 42 (bottom). Venn diagrams and top overlapping genes (middle panel). Enrichment of Gene Ontology biological process terms of overlapping genes (right panel). c Normalized counts (mean ± SEM, n = 5 mice/group) of the selected glial genes from the bRNA-seq datasets Days 1, 5, and 42 after icv FGF1 or vehicle injection. d Normalized counts (mean ± SEM, n = 5 mice/group) of Agrp, Npy, Mef2c, and Pomc expression Days 1, 5, and 42 after icv FGF1 or vehicle injection. e Targeted relative gene expression of Agrp, Npy, and Pomc. Unpaired t test, two tailed, *p = 0.04 (not adjusted, n = 5 mice/group); ns not significant. Source data are provided as a Source data file. f Threshold-free geneset enrichment analysis (Gene Ontology biological processes terms) of DEGs identified in bRNA-seq data at Days 1, 5, and 42. Point size is scaled by −log10(p value) (Bonferroni adjusted) and is colored based on the normalized enrichment score (NES). g Representative electron microscopic images of synapses of Lepob/ob mice 5 days after icv injection of either vehicle or FGF1. syn synaptic active zone, DCV dense-core vesicle, SV small light-core vesicle. Scale bar 500 nm. Quantification: dots represent mean %DCV/synapse per mouse (25 synapses per animal, n = 4/group). Unpaired t test, two tailed, *p = 0.01 (not adjusted); ns not significant. Source data are provided as a Source data file. Data are presented as mean ± SEM.
