Fig. 2: Initial optimization via SAR-by-catalog of the hit scaffold 2-based series. | Nature Communications

Fig. 2: Initial optimization via SAR-by-catalog of the hit scaffold 2-based series.

From: Structure-based evolution of a promiscuous inhibitor to a selective stabilizer of protein–protein interactions

Fig. 2

a Eight derivatives of 2 were selected for molecular docking and in vitro testing. Titration curves of b ligands on 2 μM 14-3-3β and 100 nM fluorescently-labeled ChREBP peptide, and c of 14-3-3β on 100 nM fluorescently-labeled ChREBP peptide in presence of increasing concentrations (1, 10, 100 μM) of compound 3. Data and error bars represent mean ± SEM, n = 3 replicates. Source data are provided as a Source data file.

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