Fig. 1: DNA damage induces Wnt/β-catenin activation independent of canonical Wnt receptor complex FZD/LRP.

a TOPFlash assay and immunoblotting analysis of MDA-MB-231 cells treated with Dox (2 µg/ml), CBP (10 µg/ml), and CPT-11 (10 µM) for 24 h. n = 3 independent experiments. p1 = 2.25E−05, p2 = 8.53E−05, p3 = 0.000118. b Immunoblotting analysis of total and active-β-catenin in MDA-MB-231, MDA-MB-468, and HCC1937 cells treated with Dox (2 µg/ml) for 24 h. c Immunoblotting analysis of MDA-MB-231 cells exposed to IR with increasing doses. TOPFlash assay (d) and immunoblotting analysis of β-catenin expression (e) in parental and LRP5/6 DKO HEK293A cells after Wnt3a (20 ng/ml) and Dox (2 µg/ml) treatment for 24 h. n = 3 independent experiments in d. p1 = 9.93E−06, p2 = 1.09E−05, p3 = 1.39E−05. TOPFlash assay (f) and immunoblotting analysis of β-catenin expression (g) in parental and DVL1/2/3-TKO HEK293T cells treated as in d. n = 3 independent experiments in f. p1 = 2.95E−06, p2 = 3.21E−06, p3 = 0.000104. h TOPFlash assay of MDA-MB-231 cells treated with Dox (2 µg/ml) for 24 h, with or without pretreatment of porcupine inhibitor LGK974 (100 nM). n = 3 independent experiments. p1 = 0.000107. The statistical analysis in a, d, f and h was performed by two-sided unpaired t-test and p values are indicated as ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001. Data are presented as Mean ± SD. Source data are provided as a Source Data file.