Fig. 1: Characterization and cell tropism of SARS-CoV-2 in human airway epithelia (HAE).
From: Morphogenesis and cytopathic effect of SARS-CoV-2 infection in human airway epithelial cells
a SARS-CoV-2 replication kinetics in HAE from different donors, HCoV-NL63 was used as a control (n = 3). b Transepithelial electrical resistance (TEER in Ω cm2) between the apical and basal poles was measured at each time point (n = 3). c SARS-CoV-2 infected both ciliated cells (72 h pi) and secretory cells (72 h pi). arrows: virus particles, arrowhead: cilium, asterisk: secretory vesicle, insets dashed-line squares indicate magnification of arrowed areas. d Costaining of SARS-CoV-2 N protein (green) with ciliated cell marker β-tubulin-IV (red), goblet cell marker Muc5AC (red), club cell marker CCSP (red), and ACE2 (red) positive cells. HCoV-NL63 N protein (green) staining was used as a control (72 h pi). Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (blue). Data a, b are the means ± s.d. of three independent biological replicates. Source data a–d are provided as a Source Data file.
