Fig. 2: ACC has an ethylene-independent role in pollen tube attraction and LURE1.2-eGFP localization. | Nature Communications

Fig. 2: ACC has an ethylene-independent role in pollen tube attraction and LURE1.2-eGFP localization.

From: Ethylene-independent signaling by the ethylene precursor ACC in Arabidopsis ovular pollen tube attraction

Fig. 2: ACC has an ethylene-independent role in pollen tube attraction and LURE1.2-eGFP localization.

a–f Representative images of semi-in vivo pollen tube guidance assays. Pretreatments (+water, +ACC, +C2H4) of ovules and agar beads are indicated. g Representative images of LURE1.2-eGFP localization in acs oct ovules 24 h after emasculation of stage 12c floral buds. Scale bar, 20 μm. h-k Average percentages of synergid cell (SC)-localized LURE1.2-eGFP in ovules per pistil (for ovules in which localization could be determined). Error bars show ± s.d. h No treatment; n = 9, 10, 9 pistils for WT, acs hex, acs oct, respectively. Different letters indicate significant difference (Welch ANOVA tests with Tamhane’s T2 multiple comparisons test P < 0.05, df = 2.0, W = 29.690). i Pretreatment with ACC (1 μM); n = 9 pistils per sample (two-tailed t-test, P = 0.0001 (***), t = 4.998, df = 16). j Pretreatment with C2H4 (10 ppm); n = 10 pistils per sample (two-tailed t-test, P = 0.0971 (N.S.), t = 1.750, df = 18). k Pretreatment with ACC (1 μM); n = 14 pistils for H2O, n = 17 pistils for ACC (two-tailed t-test with Welch’s correction P < 0.0001 (****), t = 5.072, df = 24.96).

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