Table 1 Quantification of pollen tube attraction in the semi-in vivo pollen tube guidance assay.

From: Ethylene-independent signaling by the ethylene precursor ACC in Arabidopsis ovular pollen tube attraction

Fig

Pollen

Stigma-style (n)

# of pollen tubes attracted to ovulesa

Probabilityc

WT

acs

WT

Neitherb

2a

WT

WT (7)

93

–

96

40

0.75–0.90

–

WT

WT (19)

227

140

–

36

<0.01

2b

WT

WT (4)

76 (+H2O)

45 (+H2O)

–

27

<0.01

–

acs

WT (10)

118

78

–

56

<0.01

–

WT

acs (10)

111

76

–

45

<0.05

 

acs

acs (11)

158

103

–

55

<0.01

2c

WT

WT (20)

215 (+H2O)

328 (+ACC)

–

151

<0.01

2d

WT

WT (13)

178 (+ambient air)

140 (+C2H4)

–

143

<0.05

–

WT

WT (8)

151 (under C2H4)

71 (under C2H4)

–

98

<0.01

2e

WT

WT (10)

215 (+H2O)

–

217 (+ACC)

146

0.90–0.95

   

# of pollen tubes attracted to agar beads

 
   

+H2O

+ACC

 

Neitherd

 

2f

WT

WT (13)

151

138

 

182

0.25–0.50

  1. aOvules of the indicated genotypes were placed in the left or right regions as in Fig. 2a–e and Supplementary Fig. 6.
  2. bPollen tubes attracted to neither left or right ovules as shown in Supplementary Fig. 6.
  3. cProbability (chi-square test) of obtaining by chance the observed variation from the expected 1:1 ratio (not including the data for Neither)
  4. dPollen tubes attracted to neither the water-soaked or ACC-soaked beads.
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