Fig. 1: Ybx1-KO NPCs had increased self-renewal.

a Schematic for BrdU labeling and analyses of mouse embryos. b IF of Sox2 and BrdU in cryosections from Ybx1-KO or sibling control embryos at E13.5. Bar, 100 μm. Quantification of IF in cryosections to determine the c number of BrdU-positive NPCs per cryosection (n = 7/genotype) or d percentage of BrdU-positive NPCs (n = 7 control or 9 Ybx1-KO biologically independent embryos) from Ybx1-KO and sibling control embryos. e Diagram of NPC isolation by CDr3 FACS and subsequent neurosphere formation and serial passages. Bar, 10 μm. Quantification of the number and area of f primary, g secondary, and h tertiary neurospheres formed by NPCs from Ybx1-KO and sibling control embryos. i Diagrams of WT full-length (FL) and mutant delNLS-YBX1 cDNAs. j Ybx1 IF in Ybx1-KO NPCs transduced with lentiviruses from empty vector, WT YBX1, or delNLS–YBX1. Inserts contain zoom-in images of representative cells. Bar, 50 μm. k YBX1 and BrdU IF in Ybx1-KO NPCs transduced with lentiviruses from empty vector, WT YBX1, or delNLS–YBX1. n = 9 images from one experiment, which was repeated twice. Bar, 50 μm. Quantification of NPCs transduced with different lentiviruses. Data are presented as mean ± SEM for c, d, and k and as violin plot of frequency distribution (lines at median and quartiles) in f–h. P values by two-tailed unpaired t test are indicated. n.s. indicate not significant. *, **, ***, and **** indicate P < 0.05, 0.01, 0.001, and 0.0001, respectively. Source data are provided in Source Data file. P values: 1c-<0.0001; 1d-0.01; 1f(bottom)-0.0005; 1g(top)-<0.0001; 1g(bottom)-0.002; 1h(top)-<0.0001, 1h(bottom)-<0.0001; 1k(empty-WT)-0.003, 1k(WT-delNLS)-0.02.