Fig. 3: Ybx1 is required for the differentiation of NPCs to neurons.
From: Ybx1 fine-tunes PRC2 activities to control embryonic brain development
a Diagram illustrating the workflow of in vitro differentiation of NPCs. b IF of Tuj1 and Gfap in Ybx1-KO and sibling control differentiating cells. c RT-qPCR with TaqMan assays of neuronal and glial genes in control and Ybx1-KO differentiating cells at day 14. n = 6/genotype. n = 10 for Neurod2 in WT, and n = 10 for Olig2 (see source data). Quantification of d relative number of Tuj1-positive neurons, e neurite length, f number of branch points in axons, g number of non-trunk branches (not connected to axons or dendrites; n = 8 images and cell numbers indicated), and h number of Gfap-positive (glial marker; n = 9 control images and 8 Ybx1-KO images) cells in Ybx1-KO and sibling control differentiating cells. Data are presented as mean ± SEM for c–h. P values by two-tailed unpaired t test are indicated. n.s. indicates not significant. *, **, ***, and **** indicate P < 0.05, 0.01, 0.001, and 0.0001, respectively. Source data are provided in Source Data file. P values: 3c(Satb2)-<0.0001, (NeuroD1)-0.0003, (NeuroD2)-0.003, (Tubb3)-0.004, (Map2)-0.009, (Dcx)-<0.0001, (NeuroG1)-0.003, (Gfap)-0.0005, (Olig1)-0.02, (Olig2)-0.01, (Cd44)-0.04, (Pdgfra)<0.0001, (Cspg4)-0.03; 3d-0.01; 3e-0.002; 3f-0.0001; 3g-0.02.
