Fig. 4: OxPHOS regulates MERCs number and function. | Nature Communications

Fig. 4: OxPHOS regulates MERCs number and function.

From: Autophagy regulates fatty acid availability for oxidative phosphorylation through mitochondria-endoplasmic reticulum contact sites

Fig. 4: OxPHOS regulates MERCs number and function.

a MOLM14 cells treated with metformin (Met, 10 mM) for 24 h were fixed and processed for electron microscopy analysis. Representative electron microscopy pictures from two independent experiments are shown. Arrows indicate MERCs. Scale bar: 1 µm. b Histograms represent the % of mitochondria that are in contact with endoplasmic reticulum per cell treated or not with Met from pictures displayed in a. Data are means ± s.e.m with each dot corresponding to one cell. c Protein components of subcellular fractions (left panel) from two independent experiments prepared from MOLM14 cells revealed by immunoblot analysis (right panel). TL: total lysate, Cy: cytosol, Mt: pure mitochondrial fraction, MERCs: mitochondria–ER contact site fraction. d Representative orthogonal confocal projections of Z sections of PLA (red signal) between VDAC1 and IP3R1 from MOLM14 cells treated or not with Met or antimycin A (AA) for 48 h (at least n = 3). Scale bar: 10 µm e Quantitative analysis of PLA signal between VDAC1 and IP3R1 from MOLM14 cells treated or not with Met or AA for 6 h, 24 h or 48 h (n = 4, one-sample t-test). Data are means ± s.e.m.

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