Fig. 3: Adhesion fibroblasts derive primarily from the viscera.
From: Elucidating the fundamental fibrotic processes driving abdominal adhesion formation
a Schematics of the PDGFRAGFP::ROSA26mTmG mouse “donor” model (left panel) and PDGFRAGFP “recipient” model (right panel). b Schematic for abdominal wall transplant model using abdominal wall from PDGFRAGFP::ROSA26mTmG mice, transplanted into PDGFRAGFP mice, followed by adhesions surgery. c Confocal imaging of representative abdominal wall transplant model mouse adhesion tissue shows a prominence of GFP+ cells within the adhesion interface, relative to GFP-mTomato+ cells, harvested at POD 14. White dotted lines mark structures as labelled in images, confocal imaging. n = 5 biological replicates. Scale bar, 100 μm. d Confocal imaging of the abdominal wall transplant model representative tissue shows PDGFRA/ASMA co-expression (PDGFRA labelled with GFP using the mTmG mouse model, IF staining for ASMA) among the cells migrating from the visceral peritoneum into the adhesion interface. White dotted lines mark structures as labelled in images, confocal imaging. n = 5 biological replicates. Scale bars, 100 μm.
