Fig. 6: Functional modulation of JUN regulates adhesion formation.

a, b Representative H&E sections for vehicle control (left panels) and JUN-inhibitor-treated (right panels), in JUN (JUN expression induced with doxycycline in all JUN mice used) (a) and wild-type (b) mice. Adhesion interfaces outlined with green dotted lines. Structures as labelled in figure. Scale bars, 100 μm. n = 5 biological replicates per condition per treatment. c Histologic scoring (as used in Fig. 1b) of adhesion tissue following in vivo inhibition of JUN using JUN inhibitor versus vehicle control in JUN (JUN expression induced with doxycycline in all JUN mice used) and wild-type mice. n = 5 biological replicates per condition per treatment. d Representative images of trichrome staining of JUN mouse vehicle control (top panel) and JUN-inhibitor-treated adhesion specimen (bottom panel). JUN expression induced with doxycycline in all JUN mice used. Adhesion interface outlined with black dotted lines. Structures as labelled in figure. n = 5 biological replicates per treatment. Conditions and structures at noted in figure panels. Scale bars, 100 μm. e Representative figures showing IF staining for phospho-JUN and PDGFRA in adhesion tissue following in vivo inhibition of JUN (using JUN inhibitor, T-5224) in JUN (JUN expression induced with doxycycline in all JUN mice used) mice. Conditions and structures at noted in figure. Scale bars, 50 μm. f Quantification of p-JUN+ cells in e. HPF high power field. n = 3 biological replicates assessed per treatment condition, datapoints represent average of multiple measures per replicate. Data and error bars represent mean ± SD. *P = 0.001, **P = 0.0001, ***P = 0.0001, unpaired two-tailed t-test. Source data are provided as a Source Data file.